alexa fluor 488 conjugated mouse anti human cd61  (Bio-Rad)

Journal: JACC: Basic to Translational Science

Article Title: Preclinical Efficacy of a Hemostatic Agent in Overcoming Dual Antiplatelet Therapy

doi: 10.1016/j.jacbts.2025.101356

Figure Lengend Snippet: In Vitro Assessment of Platelet Reactivity by Aggregometry and MF (A) Percent maximal aggregation of platelets from patients on dual antiplatelet therapy (DAPT) (n = 27) in response to adenosine diphosphate (ADP) (20 μmol/L) and TRAP-6 (10 μmol/L). Results are compared with healthy donors (n = 15). (B) Mean fluorescence intensity (FI) (n = 8 clotting events per individual) of accumulated platelets from whole blood of healthy donors vs patients on DAPT using a PDMS (polydimethylsiloxane) device attached to a type I equine collagen–coated glass slide. Digital imagines were captured using an automated fluorescent microscope system ( t = 900 seconds). (C) Photomicrograph of a fabricated collagen-coated chip and deposition of platelets labeled with Alexa Fluor 488–conjugated mouse anti-human CD61 from healthy donors or DAPT-treated patients ( t = 900 seconds). (D) Mean platelet FI (n = 8 clotting events per individual) of accumulated platelets from WB of the same individuals from using collagen-coated chips and an integrated microfluidic (MF) device. (E) Maximal thrombus area (μm 2 ) occupied by calcein-labeled platelets from healthy donors vs patients (n = 10 mice per group, 10 individuals for each of the 2 groups, 3 arteriole injuries per mouse). The central box represents the values between the 10th and 90th percentiles, and the middle line is the mean. Statistical significance was determined using Mann-Whitney U test. ∗∗∗ P < 0.001 relative to healthy donors; ∗∗∗ P < 0.001 ADP relative to TRAP-6 for patients on DAPT.

Article Snippet: The thrombin inhibitor H-D-Phe-Pro-Arg-chloromethylketone (PPACK), Alexa Fluor 488–conjugated mouse anti-human CD61, and Alexa Fluor 647–conjugated human fibrinogen were obtained from Bio-Rad and Thermo Fisher Scientific, respectively.

Techniques: In Vitro, Fluorescence, Coagulation, Microscopy, Labeling, MANN-WHITNEY

Journal: Cells

Article Title: Leukocyte-Derived Extracellular Vesicles in Blood with and without EpCAM Enrichment

doi: 10.3390/cells8080937

Figure Lengend Snippet: ACCEPT gates used for the automated enumeration of leukocytes, leukocyte-derived Extracellular Vesicles (ldEVs), platelets, and red blood cells in blood A with EpCAM enrichment and B without EpCAM enrichment.

Article Snippet: Blood was incubated with the nuclear dye Hoechst 33342 (Invitrogen, cat. # H3570), the fluorescently tagged monoclonal antibodies CD45-PerCP (clone HI30 Invitrogen, cat. # MHCD4531) recognizing leukocytes, CD235a-Alexa Fluor ® 647 (clone YTH89.1, bio-rad, MCA 506A647) antibodies recognizing erythrocytes and CD61-Alexa Fluor ® 488 (clone Y2.51, bio-rad, cat. # MCA 2588A488) antibodies recognizing platelets.

Techniques: Standard Deviation

Journal: Cells

Article Title: Leukocyte-Derived Extracellular Vesicles in Blood with and without EpCAM Enrichment

doi: 10.3390/cells8080937

Figure Lengend Snippet: Examples of objects detected in the immunofluorescence image data sets of blood samples without EpCAM enrichment. The objects fall in the ACCEPT gates, the names of which are indicated vertically. Panel ( A ) shows examples of ACCEPT thumbnails. Scale bars indicate 6.4 μm. Panel ( B ) shows scatter plots of the mean intensity of the Hoechst versus the mean intensity of CD45-PerCP and the mean intensity of CD61-Alexa 488 versus the mean intensity of CD235a-Alexa 647. Blue dots represent single events falling in the respective gate.

Article Snippet: Blood was incubated with the nuclear dye Hoechst 33342 (Invitrogen, cat. # H3570), the fluorescently tagged monoclonal antibodies CD45-PerCP (clone HI30 Invitrogen, cat. # MHCD4531) recognizing leukocytes, CD235a-Alexa Fluor ® 647 (clone YTH89.1, bio-rad, MCA 506A647) antibodies recognizing erythrocytes and CD61-Alexa Fluor ® 488 (clone Y2.51, bio-rad, cat. # MCA 2588A488) antibodies recognizing platelets.

Techniques: Immunofluorescence